| Maxime RT-PCR Pre-Mix | |||
Maxime RT-PCR Pre-Mix Cat #25131DescriptionNormal RT-PCR method is that RT- PCR procedure used by DNA polymerase for cDNA synthesis procedure are reacted in each tubes. However, this method is very inconvenient and can have cross contamination by sample¡¯s carry over. For these problems and inconvenience, Maxime RT-PCR PreMix Kit made a product that is include every container for DNA polymerase and each reaction mixture for you and can do first-strand cDNA synthesis and PCR from total RNA or mRNA template continually in a tube. High Purity Enzyme DNA Polymerase, using the anion exchange chromatography, so we can attain 95~99% of pure polymerase. Convenience The product is mixed every component. RTase, StarTaqTM DNA Polymerase, dNTP mixture, reaction buffer, and so on- in one tube for 1 rxn PCR. It contain Loading dye. So, after PCR, we can check it immediately. Reproducibility We quoted ISO 9001 and 14001, as perform special QC, we guarantee regular quality. Guarantee Date Store at -20¡ÆC for one year. iNtRON¡¯s Advantages Add only RNA template for RT. Mixed with Oligo(dT)15 primer or Random primer in PCR tube. Eliminate complicated procedure such as incubation step. Comparison with different kit  Comparison of Maxime RT-PCR PreMix Kit and Company A's RT-PCR PreMix system Kit by diagnosis of Newcastle disease (common type). 108.0EID50/0.1 ml of allantoic fluid were 10-fold dilution, then total RNA were isolated using Viral Gene- spinTM Viral DNA/RNA Extraction Kit (Cat.No. 17151). From total RNA, the synthesized first strand cDNA and PCR reaction were performed using ONE-STEP RT-PCR PreMix Kit and Maxime RT-PCR PreMix Kit. Lane M, 100bp Ladder DNA Marker Lane 1, 10-6 dilution Lane 2, 10-7 dilution Lane 3, 10-8 dilution  Comparison of Maxime RT-PCR PreMix and Company A's RT-PCR PreMix system by amplifying 570bp DNA fragment (GAPDH). Total RNA was purified from SNU-1 using easy-BLUETM Total RNA Extraction Kit(Cat. No. 17061). And then, RT-PCR reaction was performed using Maxime RT-PCR PreMix and different company¡¯s RT-PCR PreMix Kit. Lane M, 100bp Ladder DNA Marker Lane 1, 2ng total RNA Lane 2, 200pg total RNA Lane 3, 20pg total RNA Lane 4, 2pg total RNA Lane 5, 200fg total RNA
|
| HOT SALE |
I PFU 250U without DNTp's |
| Intron products at Talron |
|
 |