| Maxime RT-PCR Pre-Mix (Randome Primer) |
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Maxime RT-PCR Pre-Mix (Random Primer) Cat # 25082
Description
Maxime RT PreMix Kit is the product what is mixed every component for
cDNA synthesis in each tube for 1 rxn PCR. Maxime RT PreMix Kit has two kinds of product,
that is, oligo (dT)15 primer and random primer- for choose the product for each using type.
The product that finished a cDNA synthesis reaction can do PCR directly by using
Maxime PCR PreMix Kit.
High Purity Enzyme
DNA Polymerase, using the anion exchange chromatography,
so we can attain 95~99% of pure polymerase.
Convenience
Maxime RT-PreMix Kit is mixed every component. RTase, dNTP mixture,
reaction buffer, Oligo dt or Random primer and so on – in one tube for 1 rxn PCR.
Reproducibility
We quoted ISO 9001 and 14001, as perform special QC, we guarantee regular quality
Guarantee Date
Store at -20 ¡ÆC for one year.
iNtRON¡¯s Advantages
Add only RNA template for RT.
Mixed with Oligo(dT)15 primer or Random primer in PCR tube.
Eliminate complicated procedure such as incubation step.
Protocol comparison with different company

Comparison with different kit

Total RNA was purified from mouse cells using easy-BLUETM Total RNA Extraction Kit
(Cat. No. 17061). And then, the first strand of cDNA was synthesized using and
Maxime RT PreMix Kit (Oligo dT Primer) and company¡¯s A RT PreMix Kit.
After diluting the cDNA mixture as indicates, the PCR reaction was performed using
Maxime PCR PreMix Kit
Comparison of Maxime PCR PreMix (Oligo dT Primer) and Company A's RT PreMix system by amplifying 570bp DNA fragment (GAPDH).
Lane M, 100bp Ladder DNA Marker Lane 1, (1/2)3 diluted cDNA
Lane 2, (1/2)4 diluted cDNA Lane 3, (1/2)5 diluted cDNA
Lane 4, (1/2)6 diluted cDNA Lane 5, (1/2)7 diluted cDNA
Created on 08/01/2005 03:28 AM by admin
Updated on 08/25/2005 05:41 AM by admin
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